Protistology • 15 (3), 170–189 (2021) |
A possible molecular mechanism of Chlamydomonas reinhardtii adaptation to different trophic conditions |
Roman Puzanskiy1,2, Daria Romanyuk3, Alexey Shavarda1,2, Vladislav Yemelyanov1 and Maria Shishova1
1 Saint Petersburg State University, Saint Petersburg, 199034, Russia
| Submitted August 28, 2021 | Accepted September 22, 2021 | Summary
Microalgae are able to metabolize exogenous organic substrates. Differences
in sets of available sources of energy and carbon cause various metabolic and
physiological alterations. To characterize those in Chlamydomonas reinhardtii, we
compared cells fed with acetate in batch cultures of CC-124 (wt-light) and CC-981
(mutant deficient in phosphoribulokinase, incapable to fix CO2), growing under
illumination (prk-light) or in the darkness (prk-dark). The growth intensity of the
wt-light culture was higher only at the beginning of the culture cycle. Its final density
only slightly exceeded such of prk-light ones. The growth rate and final density of
the prk-dark culture were several times lower.
Key words: acetate, batch culture, Chlamydomonas, heterotrophic, metabolomics, mixotrophic, prk1
Address for correspondence: Maria Shishova. Biological Faculty, St. Petersburg State University, Universitetskaya Emb. 7/9, 199034 St. Petersburg, Russia; e-mail: mshishova@mail.ru |
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