Protistology 2 (2) 123-129 (2001) Heat shock protein of 70 kDa in Amoeba proteus
Yulia I. Podlipaeva
Laboratory of Cytology of Unicellular Organisms, Institute of Cytology, Russian Academy of Sciences,
St. Petersburg, Russia
Summary
Heat shock protein of 70 kDa (HSP70) was revealed in the cells of Amoeba proteus strain Da by immunoblotting with the use of poly- and
monoclonal anti-HSP70 antibodies. This protein can bind ATP and GTP according to ATP-agarose chromatography data. The untreated cells
contained surprisingly high level of HSP70. The one hour heating of the amoeba culture at 37° C did not cause the induction of HSP70, but
resulted in pronounced decrease of its level in 4 hrs after the heat shock. This phenomenon might be connected with the utilization of HSP70
reserves in stress-conditions to protect sensitive protein structures of the cell. After using the monoclonal anti-HSP70 antibodies the increase
in the HSP70 content was revealed in 4 hrs after one hour heating of amoeba cells at 32° C - the temperature close to the borders of
temperature tolerant range (TTR) of Da amoeba strain. It looks like the temperatures causing the induction of HSPs depend upon the strain
(species) TTR, presumably even indicating its natural borders.
Key words: Amoeba proteus, heat shock protein
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