Protistology 3 (2) 126–135 (2003) The taxonomic position of Klosteria bodomorphis gen. and sp. nov. (Kinetoplastida) based on ultrastructure and SSU rRNA gene sequence analysis
Sergey I. Nikolaev 1, Alexander P. Mylnikov 2, Cedric
Berney 3, Jose Fahrni 3, Nikolai Petrov 1 and Jan Pawlowski 3
1 A. N. Belozersky Institute of Physico-Chemical Biology,
Department of Evolutionary Biochemistry, Moscow State University, Moscow, Russia
Summary
A small free-living marine bacteriotrophic flagellate Klosteria bodomorphis gen. and sp. nov. was investigated by electron
microscopy and molecular methods. This protist has paraxial rods of typical bodonid structure in the flagella, mastigonemes on the
anterior flagellum, two nearly parallel basal bodies and discoid mitochondrial cristae. The flagellar pocket and
cytostome/cytopharynx complex are supported by two microtubular roots and reinforced microtubular band (mtr). These features
confirm that K. bodomorphis is a bodonid related to Bodo. However, the presence of a layer of dense glycocalyx on the flagella and
one battery of cylindrical trichocysts with reticular walls makes it more similar to Rhynchobodo/Phyllomitus, although this flagellate
characterized by pankinetoplsty. Phylogenetic analysis using the SSU rRNA gene is congruent with the ultrastructural studies and
strongly confirms the close relationship of K. bodomorphis to the genus Rhynchobodo within the order Kinetoplastida. Key words: Bodonidae, Klosteria bodomorphis, evolution, 18S rDNA, phylogeny
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