Tenuibiotus yeliseii sp. nov., a new species of Macrobiotidae (Tardigrada: Eutardigrada) from Svalbard, Norway, with discussion of taxonomic criteria within the genus and its phylogeny

A.Yu. Tsvetkova & D.V. Tumanov

Abstract. We describe Tenuibiotus yeliseii sp. nov., a new tardigrade species from Svalbard, using morphological and morphometric analyses conducted with the use of light and scanning electron microscopy, as well as genetic analyses based on four molecular markers (three nuclear, 18S rRNA, 28S rRNA, ITS-2, and one mitochondrial, COI). A phylogenetic analysis of the genus Tenuibiotus Pilato et Lisi, 2011 is conducted using new data. In addition, the taxonomic significance of gibbosities on leg IV as a key character for species identification in Tenuibiotus is discussed. A key to the species of Tenuibiotus is proposed.

Key words: Arctic, taxonomy, morphology, phylogeny, key, Tardigrada, Macrobiotoidea, Macrobiotidae, Tenuibiotus, new species

Zoosystematica Rossica, 2024, 33(1): 28–47 ▪ Published online 24 April 2024

https://doi.org/10.31610/zsr/2024.33.1.28 ▪ Open full article

Electronic supplementary materials

Electronic supplementary material 1

Primers and PCR programs used for amplification of four DNA fragments sequenced in the study. File format: PDF.

Electronic supplementary material 2

Complete list of sequences used in the molecular phylogenetic analysis. File format: PDF.

Electronic supplementary material 3

Final alignment used for the phylogenetic analyses. File format: FST, as 7Z archive.

Electronic supplementary material 4

Results of the selection of substitution model for redefined partitions. File format: TXT.

Electronic supplementary material 5

Measurements of animals and eggs. File format: XLSX.

Electronic supplementary material 6

Matrices of p-distances for Tenuibiotus species. File format: XLSX.

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