Express technique to prepare permanent collection slides of nematodes

Abstract. A simple method of 1-2 hours technique to prepare permanent collection slides is described and illustrated (normally the slide preparation takes 1-2 weeks). The method may be recommended for cases when the express identification is needed: in quarantine and plant protection services, and as an auxiliary method in molecular and applied investigations, to prove reliability of the species identification after years and future taxonomic changes. The method includes killing, fixation, processing in glycerin, and preparation of slides using an Eppendorf tube at high temperature (60-95 °C).