무당벌레 (Harmonia axyridis) 난황단백질의 부분정제와 특성
(Partial Purification and characterization of Vitellins of the Ladybeetle , Harmonia axyridis)
http://www.riss.kr/link?id=A3167760
- 제어번호 : 3167760
- 저자명 : 박용철 ,홍옥기
- 학술지명 : 한국 곤충학회지(Korean journal of entomology)
- 권호사항 : Vol.26 No.1 [1996]
- 발행처 : 한국곤충학회
- 발행처 URL : http://www.korentsoc.org/
- 자료유형 : 학술저널
- 수록면 : 29-38(10쪽)
- 언어 : Korean
- 발행년도 : 1996년
- KDC : 495.000
-
소장기관 :
- 경북대학교 중앙도서관
- 서울대학교 중앙도서관
- 연세대학교 중앙도서관
- 영남대학교 중앙도서관
- 중단사유 : ※ 판매처에서 원문제공을 중단하였으니, 학술지명을 클릭하여 [복사/대출] 서비스를 이용해 주시기 바랍니다.
-
- Vitellins in the eggs of the ladybeetle, Harmonia
axyridis, were composed of 4 subunits; large molecular weights, Vn1
(136,000) and Vn2 (132,000), and small molecular weights, Vn3 (50,000) and
Vn4 (45,000). On Western blot, vitellins were detected from the egg, ovary
and hemolymph of female adult, but not from the fat body and hemolymph of
male. In SDS-PAGE, large molecular weights Vn1 and Vn2 were comigrated but
separated when protein concentration was low. Vitellins were partially
purified. from eggs by four step procedures including n-buatnol and 10%
Polyethylene Glycol (PEG, 10,000) treatment. Antibodies were made against
the partially purified vitellins, Vn1 and 2, Vn3, and Vn4 from rabbits.
There were no antibody cross reactions between large molecular weight
subunits, Vn1 and 2, and small molecular weight subunits, Vn3 and Vn4.
Subunits of vitellins in the ladybeetles's eggs were present as a tightly
bound complex which was dissociated in the presence of SDS and Triton X-100,
but not in the high salt concentration, 0.8 M NaCl.
- 초록전체보기
- Vitellins in the eggs of the ladybeetle, Harmonia
axyridis, were composed of 4 subunits; large molecular weights, Vn1
(136,000) and Vn2 (132,000), and small molecular weights, Vn3 (50,000) and
Vn4 (45,000). On Western blot, vitellins were detected from the egg, ovary
and hemolymph of female adult, but not from the fat body and hemolymph of
male. In SDS-PAGE, large molecular weights Vn1 and Vn2 were comigrated but
separated when protein concentration was low. Vitellins were partially
purified. from eggs by four step procedures including n-buatnol and 10%
Polyethylene Glycol (PEG, 10,000) treatment. Antibodies were made against
the partially purified vitellins, Vn1 and 2, Vn3, and Vn4 from rabbits.
There were no antibody cross reactions between large molecular weight
subunits, Vn1 and 2, and small molecular weight subunits, Vn3 and Vn4.
Subunits of vitellins in the ladybeetles's eggs were present as a tightly
bound complex which was dissociated in the presence of SDS and Triton X-100,
but not in the high salt concentration, 0.8 M NaCl.
-
데이터가 존재 하지 않습니다.
