Архив выпусков

The most important parameters necessary for the creation of population models for threehost species with long-term life cycles are discussed with an example of ticks Ixodes persulcatus and I. ricinus. In these species, specimens of the same biological age may belong to different age cohorts and their calendar age may differ by several months or even years. Accurate estimation of the calendar age of separate individuals is dofficult; it is based on the extrapolation by its possible biological age and by belonging to the certain age cohort of a natural population. Population models that can predict simultaneous abundance of activated hungry specimens of all the three developmental stages and probability of host-finding in hyngry ticks during questing period possess the prognostic value. Daily mortality of ticks of different developmental stages and phases of each stage (questing, feeding, preparation for molting, and diapause) must also be known. The abundance of questing hungry ticks in the ecosystem is determined by the balance between recruitment of the population with new individuals, their selection by hosts, dying of ticks from starvation, and consumption of ticks by predators. At present, unfortunately, only some of these parameters are known rather sufficiently.

Data obtained during feeding of Citellophilus tesquorum altaicus Ioff, 1936 infested females and males (Siphonaptera: Ceratophyllidae), the main vectors of plague in Tuva natural plague locus, on the natural host and laboratory animal was analyzed. It was found that sexual differences in fleas depended on the type of the host. Females fed more actively on the longtailed ground Citellus undulatus than on white mouse. Alimentary activity of males on these animals was similar. Higher mortality of fed females and males was noted during feeding on mice. Frequency of formation of the "block" and transmission of the pathogen in males was higher during bloodsucking on the ground squirrel; in females, during feeding on mice. Thus, differences in the transmission of the plague pathogen, revealed in laboratory on white mice, can be quite different in nature. So, extrapolation of experimental data on natural processes of interrelations between plague pathogen and ectoparasites must be performed taking into account revealed peculiarities.

Trematode fauna of waterfowl birds of Karelia comprises 23 species; 8 of them are described from the region for the first time. Descriptions and figures of Urogonimus macrostomus, Neoeucotyle zakharovi, Hypoderaeum conoideum, Echinostoma robustum, Orchipedum tracheicola, Prostogonimus cuneatus, P. ovatus, and P. rarus are given.

Earlier analysis of a seasonal succession of fish parasites assemblage was, as a rule, performed in May-October. At the same time, winter season can be important in seasonal dynamics of the assemblage. In the present work, quantitative analysis of complete seasonal dynamics of species structure of fish assemblage parasites with an example of the adult minnow of the river Pechora was performed. The new stable state of parasites assemblage of the minnow, corresponding to winter season of its existence, is revealed. Irrespective of population density or abiomass of parasitic assemblages of the minnow, obtained dynamic phase portraits well reflect their seasonal succession.

The results of our own long-term helminthological investigations (1980—2010) of wolves in Belorussian Polesie are presented. 87.2 % of wolves were infected by helminths according to helminthological autopsy, and 98.5 % by coproscopical data. 26 species of helminths were found: 5 trematode species, 9 cestode species, 11 nematode species and 1 acanthocephalan species. The wolf is a new host for nematode Dirofilaria immitis (Leidy, 1856) in Belarus.

Species composition and structure of the strongylid community was studies on helmin-thological material collected from 162 domestic horses from 11 regions of Ukraine by the in vivo method. Animals were treated with anthelmintic drug "Univerm" (0.2% aversectin C, Russia). Faecal samples (200 g each) were collected from every horse at 24, 36, 48 and 60 hours after treatment; all nematodes expelled (90.851 specimens) were collected and identified. Thirty-three strongylid species from 12 genera (8 species of subfamily Strongylinae and 25 — of Cyathostominae) were found in domestic horses in Ukraine. Cyathostominae dominated in the strongylid community; they were found in 100% horses and composed 98.21% of community. "Core" of the strongylid community was composed by 7 cyathostome species. Decreasing of proportion of Strongylinae in the community for last 40 years was registered; strongylines were found in 37.6% of horses and composed 1.25% of community. Maximal prevalence was 20.98% (Strongylus vulgaris). Bray-Curtis cluster analysis revealed high similarity of strongylid communities in horses from various regions of Ukraine. Difference in general structure of strongylid communities of horses from different horse-keeping conditions was established. Horses from farms with stable-paddock keeping conditions had bimodal strongylid community structure; while horses from stable-pasture keeping conditions possessed multimodal community structure.

A number of microscopic techniques and dyes are available to diagnose microsporidi-an infections in invertebrate and vertebrate hosts. Among these, DNA-specific fluorochrome DAPI is widely used to stain DNA in prokaryotic and eukaryotic cells, alone or in combination with other histochemical or fluorescent dyes. Moreover, this dye also binds to membraneous structures and protein complexes. In our studies, DAPI was used to stain spores of microsporidia infecting orthopteran, coleopteran, dipteran and lepidopteran insect hosts. DAPI staining of diplokarya helped to discriminate the Nosema-like microsporidian spores from spore-shaped bodies lacking this characteristic staining. It was found, moreover, that non-DNA staining occurred in many cases and other components of the spores were stained: the exospore, the cytoplasm, the extruded polar filament and the polaroplast. Staining of these structures was feeble as compared to DNA and in most cases did not interfere with nuclear apparatus staining. Feebly stained cytoplasm and exospore clearly indicated unstained zone of endospore, making it easier to diagnose both mono- and diplokaryotic spores. Staining of extruded polar filament allowed to demonstrate viability and to observe some stages of extrusion process of microsporidian spores.

---