Protistology 2 (2) 96–104 (2001) Molecular taxonomy of virus-sensitive Chlorella sp. - symbionts of Paramecium bursaria
K .V. Kvitko, A. V. Migunova, D. V. Karelov and M. Ju. Prokosheva
Laboratory of Microbiology, Biological Research Institute of St. Petersburg State University, St.
Petersburg, Russia, and
Summary
Collection of virus-sensitive Chlorella-like algae isolated from a ciliate Paramecium bursaria is described. Differences are revealed in
surface antigens and polymerase chain reaction (PCR) patterns of two groups of virus-sensitive Chlorella strains from "northern" and
"southern" populations of hosts. A correlation between virus-sensitivity, serological characteristics, and UPPCR-patterns of the zoochlorellae
strains DNA is shown. We have proposed considering this alga as the Chlorella paramecii group of two contrasting ("northern" and
"southern") ecotypes, each of them having a specific uniform serotype characteristic. This subdivision coincides with two groups of practically
the same set of Chlorella sp. strains differing by 8 isoenzyme patterns and described in our previous paper (Linz et al., 1999).
Key words: zoochlorellae, Chlorella vulgaris / Ch. sorokiniana, Chlorella paramecii, molecular taxonomy, PBCV-virus,
virus-sensitivity of Paramecium bursaria symbionts, isoenzymes, serological characterization, agglutination, precipitation,
immunoblotting, surface antigen specificity, UP-PCR-patterns, stability of symbionts, DNA base composition, GC% DNA, DNA homology
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