Protistology 4 (2) 185-190 (2005)
A simple mass culture of the amoeba Chaos carolinense: revisit
Olivia Li Ling Tan, Zakaria Ali Moh. Almsherqi and Yuru Deng
Department of Physiology, Faculty of Medicine, National University of Singapore,
Singapore
Summary
The free-living amoeba Chaos carolinense used to be a very popular laboratory object in the 1950s-60s
and is still one of the favorite classroom objects for demonstration of amoeboid movement. Large-scale production of this
organism is often needed for various experimental purposes. We have established a reproducible, simple mass culture method
specifically suitable for C. carolinense, with feeding on only one food organism (Paramecium multimicronucleatum),
and discuss the problems that have been encountered during establishing and maintaining of this culture.
Key words: amoeba, Chaos carolinense, mass culture, Paramecium
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