Protistology 8 (1) 3–7 (2013) |
New method for cell cycle synchronization in Amoeba proteus culture |
Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia Summary A method to synchronize cell cycle in the culture of Amoeba proteus is proposed. Amoeba cells were kept in quail egg albumen and thus having been fed by the pinocytosis, then thoroughly washed from the protein and examined at stained slides in different periods of time after washing. It appeared that practically all the cells on the slide corresponding to certain time after feeding/washing underwent the same stage of cell cycle – either interphase or one of the phases of mitosis depending on time. The method of amoebae feeding by the pinocytosis also allows avoiding negative effect of food remains in the cell cytoplasm, thus providing the higher quality of stained slides. Key words: Amoeba proteus, cell cycle, mitosis, pinocytosis, synchronization Address for correspondence: Andrew Goodkov. Institute of Cytology, Russian Academy of Sciences, Tikhoretsky Ave. 4, St. Petersburg, 194064, Russia; e-mail: pelgood1@gmail.com |
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