PreparationNervous and muscular systems in the anterior end of a polychaete Schistomeringos japonica (Annenkova, 1937) (Annelida, Polychaeta). |
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Collection ZIN-StaV-0-1 |
Species: Schistomeringos japonica (Annenkova, 1937) Annelida Preparation Type: whole mount Prepared: V.V. Starunov Description: Musculature is stained by TRITC-conjugated phalloidin. Nervous system is labeled using antibodies against acetylated tubulin and serotonin. Method: acetylated alpha-tubulin, F-actin in muscle fibers (Phalloidin), serotonin Method Description: The staining protocol is described in detail in: Starunov V. V. et al. A metameric origin for the annelid pygidium? // BMC evolutionary biology. – 2015. – V. 15, No 1. – P. 25. http://bmcevolbiol.biomedcentral.com/articles/10.1186/s12862-015-0299-z Institution: Zoological Institute RAS |
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Preparation ZIN-StaV-0-1 |
Description: Muscles are shown in red, ciliary structure and nerve fibers immunoreactive to acetylated tubulin are green. Autofluorescent chaete and serotonin-immunoreactive elements are cyan. Organs: сentral nervous system, cerebral brain or its part, ganglion / ganglia, nerve, peripheral nervous system Methods: Immunohistochemistry - serotonin, |
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