Preparation

Metathoracic ganglion of a 4 stage larva of the migratory locust Locusta migratoria (Insecta, Orthoptera).

In
Collection 30

Collection
Number

ZIN-TVK-LNS-1-3

Species: Locusta migratoria (Linnaeus, 1758)    Arthropoda

Preparation Type: whole mount

Prepared: T.V. Kuznetsova

Description: Isolated metathoracic ganglion, stained by anterograde transport of a solution of cobalt chloride through the motor branch of the 4th nerve of the metathoracic ganglion. The branch innervates the bifunctional 120th muscle that participates in walking and flight.

Method: retro-anterograde transport of cobalt chloride method

Method Description: 1.5% solution of cobalt chloride was used. Cobalt chloride was administered by passive transport for 3 days at 4-5 °С and precipitated by ammonium sulphide. The staining was additionally enhanced by silver impregnation.

Institution: Zoological Institute RAS

 

 

 

Series
of images

Motoneurons in the metathoracic ganglion of a 4 stage larva migratory locust Locusta migratoria (Insecta, Orthoptera).

Preparation

ZIN-TVK-LNS-1-3

Description: Nervous elements in the metathoracic ganglion of a locust involved in the innervation of the bifunctional muscle 120. Nervous fibers and 4 motoneurons are visible.

Organs: сentral nervous system, ganglion / ganglia, neuron(s), nerve, larval nervous system

Methods: Retrograde and anterograde tracing techniques, extra- and intracellular labeling of neurons - retro-anterograde transport of cobalt chloride method

Series Details...