Preparation

Short axon, aspine, stellate neuron dorsal area telencephalon of a shark Squalus acanthias Linnaeus, 1758 (Chordata, Chondrichthyes, Selachii)

In
Collection 19

Collection
Number

SPSU-ODK-PIS-2-1

Species: Squalus acanthias Linnaeus, 1758    Pisces

Preparation Type: 60-100 µm thick celloidin sections

Prepared: D.K. Obukhov

Description: Frontal (transverse) celloidin section through an area of a telencephalic hemisphere of the spiny dogfish Squalus acanthias Linnaeus, 1758 impregnated with silver salts by the Golgi method.

Method: Golgi method of silver impregnation of nervous elements

Method Description: Frontal celloidin sections. Golgi impregnation.
The staining protocol is described in detail in the handbooks of microscopy methods: Mulisch, M., Welsch, U. Romeis - Mikroskopische Technik, 18. Auflage. - Heidelberg: Spektrum, 2010. - 568 p.

Institution: Saint Petersburg State University

 

 

 

Series
of images

Central nervous system, Brain, Telencephalon, Neurons, Chondrichthyes, Selachii

Preparation

SPSU-ODK-PIS-2-1

Description: Frontal (transverse) celloidin section through a telencephalic hemisphere of the brain of a cartilaginous fish (spiny dogfish) impregnated with silver salts by the Golgi method. Dorsal region of the pallium. The section shows a separately stained short-axon, spineless, stellate nerve cell with a system of dendritic (d) and axonal (ax) arborizations. Note a rich network of axonal collaterals (aхc) and the lack of spines on the dendrites. According to the classification of E. Ramon-Molinera, these neurons belong to a group of highly specialized, idiodendritic cells, which shows a high level of neuronal organization of the brain in some sharks. It is important to note that this kind of cells are found only in the pallium of higher vertebrates (birds and mammals).

Organs: cerebral brain or its part, сentral nervous system

Methods: Neurohistology - Golgi method of silver impregnation of nervous elements

Series Details...