Species: Platynereis dumerilii Audouin & Milne Edwards, 1834    Annelida

Preparation Type: whole mount

Prepared: V.V. Starunov

Description: Musculature is stained by TRITC-conjugated phalloidin. Nervous system is labeled using antibodies against acetylated tubulin and FMRFamide.

Method: acetylated alpha-tubulin, F-actin in muscle fibers (Phalloidin), FMRFamide

Method Description: The preparation was made using the technique described in: V. V. Starunov et al., A metameric origin for the annelid pygidium? BMC Evol. Biol. 15, 1–17 (2015). (http://www.biomedcentral.com/1471-2148/15/25)

Institution: Zoological Institute RAS

Description: Muscles are shown in red, FMRFamide-positive immunoreactivity is green. Nerve fibers, labelled by acetylated tubulin, are shown in blue.

Organs: сentral nervous system, cerebral brain or its part, ganglion / ganglia, Sensory organ

Methods: Immunohistochemistry - FMRFamide,
Immunohistochemistry - acetylated alpha-tubulin,
Histochemistry - F-actin in muscle fibers (Phalloidin)