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Total preparation of a Plumatella repens zooid, stained with FMRFamide antibody and phalloidin.

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Preparation

ZIN-KVS-0-2

Storage
place

ZIN RAS

Collection: Nervous and muscle systems of Bryozoa

Species: Plumatella repens (Linnaeus, 1758)    Bryozoa

Description: Plumatella repens. Visceral and peripheral nervous systems at the base of the lophophore and in the wall of the cystide stained with antibodies to FMRFamide. The series shows that the majority of nerve fibers run within the intestinal wall and the wall of the cystide parallel to the apico-basal axis of the zooid. The preparations stained with phalloidin show the differences in muscle arrangement in different regions of the zooid and in orientation of muscle fibers in the walls of the cystide and intestine.

Comments: none

Organs: neural tract, peripheral nervous system, visceral nervous system

Methods: Histochemistry - F-actin in muscle fibers (Phalloidin),
Immunohistochemistry - FMRFamide

Publications: Шунькина К.В., Старунов В.В., Зайцева О.В., Островский А.Н. Серотонин и FMRFамид- иммунореактивные элементы в нервной системе пресноводных мшанок (Bryozoa: Phylactolaemata) // Доклады Академии Наук. – 2013. – Т. 451. – №4. – С. 474-477.Шунькина К.В., Старунов В.В., Зайцева О. В., Островский А.Н. Сравнительная нейроморфология лофофора и стенки тела трех видов пресноводных мшанок (Bryozoa, Phylactolaemata) //Зоологический журнал. – 2014. – T. 93. – №3. – С. 497-507.Shunkina K.V., Zaytseva O.V., Starunov V.V., Ostrovsky A.N. Comparative morphology of the nervous system in three phylactolaemate bryozoans // Frontiers in Zoology 2015, 12:28 doi:10.1186/s12983-015-0112-2

Microscope: Leica TCS SPE

Stacks: 

Musculature (phalloidin)
FMRFamide

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