PreparationSerotonergic nervous system of the lophophore of the freshwater bryozoan Fredericella sultana (Bryozoa, Phylactolaemata) |
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Collection ZIN-KVS-0-3 |
Species: Fredericella sultana Blumenbach, 1779 Bryozoa Preparation Type: whole mount Prepared: K.V. Shunklina Description: Whole mount of a Fredericella sultana zooid, stained with 5HT, α-tubulin antibodies and phalloidin. Method: alpha-tubulin, F-actin in muscle fibers (Phalloidin), serotonin Method Description: The staining method and protocol are described in detail in: Shunkina, K. V., Zaytseva, O. V., Starunov, V. V., & Ostrovsky, A. N. (2015). Comparative morphology of the nervous system in three phylactolaemate bryozoans. Frontiers in zoology, 12(1), 28. http://www.frontiersinzoology.com/content/12/1/28 Institution: Zoological Institute RAS |
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Preparation ZIN-KVS-0-3 |
Description: Fredericella sultana. Central nervous system and the nervous system of the lophophore stained with antibodies to serotonin. The lophophore of the zooid was imaged from the anal side. Deep in the preparation in the region of the cerebral ganglia, there is a clearly visible nerve net of serotonergic fibers. An additional staining with antibodies to alpha-tubulin shows the arrangement of nerves that innervate the base of the lophophore and the tentacles of the zooid. Phalloidin staining shows the positional relationships between the muscle and nerve fibers in the lophophore. Organs: neural tract, ganglion / ganglia, neuron(s), peripheral nervous system, musculature innervation / musculature, receptor cell(s) Methods: Histochemistry - F-actin in muscle fibers (Phalloidin), |
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