Collection: Nervous and muscle systems of Bryozoa

Species: Fredericella sultana Blumenbach, 1779    Bryozoa

Description: Fredericella sultana. Central nervous system and the nervous system of the lophophore stained with antibodies to serotonin. The lophophore of the zooid was imaged from the anal side. Deep in the preparation in the region of the cerebral ganglia, there is a clearly visible nerve net of serotonergic fibers. An additional staining with antibodies to alpha-tubulin shows the arrangement of nerves that innervate the base of the lophophore and the tentacles of the zooid. Phalloidin staining shows the positional relationships between the muscle and nerve fibers in the lophophore.

Comments: none

Organs: neural tract, ganglion / ganglia, neuron(s), peripheral nervous system, musculature innervation / musculature, receptor cell(s)

Methods: Histochemistry - F-actin in muscle fibers (Phalloidin),
Immunohistochemistry - serotonin,
Immunohistochemistry - alpha-tubulin

Publications: 

1. Шунькина К.В., Старунов В.В., Зайцева О.В., Островский А.Н. Серотонин и FMRFамид- иммунореактивные элементы в нервной системе пресноводных мшанок (Bryozoa: Phylactolaemata) // Доклады Академии Наук. – 2013. – Т. 451. – №4. – С. 474-477.
2. Шунькина К.В., Старунов В.В., Зайцева О. В., Островский А.Н. Сравнительная нейроморфология лофофора и стенки тела трех видов пресноводных мшанок (Bryozoa, Phylactolaemata) //Зоологический журнал. – 2014. – T. 93. – №3. – С. 497-507.
3. Shunkina K.V., Zaytseva O.V., Starunov V.V., Ostrovsky A.N. Comparative morphology of the nervous system in three phylactolaemate bryozoans // Frontiers in Zoology 2015, 12:28 doi:10.1186/s12983-015-0112-2.

Microscope: Leica TCS SP5

Stacks: 

Serotonin and phalloidin
Serotonin
Phalloidin
Tubulin
Serotonin and tubulin
Serotonin, tubulin and phalloidin